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Obio Technology Corp Ltd negative control nc sirna
Negative Control Nc Sirna, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+nc+sirna/control+negative/pm42001119-127-15-19
Average 86 stars, based on 1 article reviews
negative control nc sirna - by Bioz Stars, 2026-10
86/100 stars

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Plasmid Preparation:

Article Title: Endothelial cell-derived IGFBP7 suppresses angiogenesis and tumor progression in colorectal cancer via the VAPA-TGF-β1 pathway.
Article Snippet: HMEC-1 cells were treated with the Golgi transport inhibitor (BD Biosciences, Cat# 554724, 1 μl/ml) or GW4869 (GLPBIO, Cat# GC19186, 10 μM) for 6 hours. .. After treatment, the culture supernatants and cells were collected for subsequent experiments. siRNA and Plasmid Transfection IGFBP7 siRNA and negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China), and siRNA transfection of HMEC-1 cells and HUVEC cells was performed according to the manufacturer’s protocol. .. Tumor cells were incubated with MG132 (20 μM, Cat# HY-13259, MedChem Express, USA) for 5 AR TIC LE IN PR ES S 9 hours or Bafilomycin A1 (20 μM) for 6 hours Subsequently, the cells were harvested for Western blot analysis.

Transfection:

Article Title: Endothelial cell-derived IGFBP7 suppresses angiogenesis and tumor progression in colorectal cancer via the VAPA-TGF-β1 pathway.
Article Snippet: HMEC-1 cells were treated with the Golgi transport inhibitor (BD Biosciences, Cat# 554724, 1 μl/ml) or GW4869 (GLPBIO, Cat# GC19186, 10 μM) for 6 hours. .. After treatment, the culture supernatants and cells were collected for subsequent experiments. siRNA and Plasmid Transfection IGFBP7 siRNA and negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China), and siRNA transfection of HMEC-1 cells and HUVEC cells was performed according to the manufacturer’s protocol. .. Tumor cells were incubated with MG132 (20 μM, Cat# HY-13259, MedChem Express, USA) for 5 AR TIC LE IN PR ES S 9 hours or Bafilomycin A1 (20 μM) for 6 hours Subsequently, the cells were harvested for Western blot analysis.

Article Title: Targeting FGG alleviates cholestatic fibrosis by inhibiting hepatic stellate cell activation and regulating macrophage homeostasis.
Article Snippet: .. For gene knockdown, cells at 70–80% confluence were transfected with 50nM human FGG-specific siRNA or negative control (NC) siRNA (OBiO Technology), with or without concurrent stimulation by 5ng/mL TGF-β1 (MCE, Shanghai, China). ..

Article Title: Endothelial cell-derived IGFBP7 suppresses angiogenesis and tumor progression in colorectal cancer via the VAPA-TGF-β1 pathway
Article Snippet: Tumor cells were treated with recombinant IGFBP7 protein (R&D Systems, Cat# 1334-B7) for 48 h. After treatment, the culture supernatants and cells were collected for subsequent experiments. .. IGFBP7 siRNA and negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China), and siRNA transfection of HMEC-1 cells and HUVEC cells was performed according to the manufacturer’s protocol. ..

Negative Control:

Article Title: Endothelial cell-derived IGFBP7 suppresses angiogenesis and tumor progression in colorectal cancer via the VAPA-TGF-β1 pathway.
Article Snippet: HMEC-1 cells were treated with the Golgi transport inhibitor (BD Biosciences, Cat# 554724, 1 μl/ml) or GW4869 (GLPBIO, Cat# GC19186, 10 μM) for 6 hours. .. After treatment, the culture supernatants and cells were collected for subsequent experiments. siRNA and Plasmid Transfection IGFBP7 siRNA and negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China), and siRNA transfection of HMEC-1 cells and HUVEC cells was performed according to the manufacturer’s protocol. .. Tumor cells were incubated with MG132 (20 μM, Cat# HY-13259, MedChem Express, USA) for 5 AR TIC LE IN PR ES S 9 hours or Bafilomycin A1 (20 μM) for 6 hours Subsequently, the cells were harvested for Western blot analysis.

Article Title: Targeting FGG alleviates cholestatic fibrosis by inhibiting hepatic stellate cell activation and regulating macrophage homeostasis.
Article Snippet: .. For gene knockdown, cells at 70–80% confluence were transfected with 50nM human FGG-specific siRNA or negative control (NC) siRNA (OBiO Technology), with or without concurrent stimulation by 5ng/mL TGF-β1 (MCE, Shanghai, China). ..

Article Title: Helicobacter pylori -induced aberrant methylation of ID4 mediated by DNMT3B drives gastric cancer progression via DEC1-SHH signaling pathway
Article Snippet: .. The ID4 small interfering RNA (siRNA) or respective negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China). ..

Article Title: Helicobacter pylori -induced aberrant methylation of ID4 mediated by DNMT3B drives gastric cancer progression via DEC1-SHH signaling pathway
Article Snippet: .. The DNMT3B and KLF5 small interfering RNA (siRNA) or respective negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China). .. The siRNAs were transfected into cells utilizing Opti-MEM and Lipofectamine 2000 transfection reagent (Invitrogen, CA, USA) at a concentration of 100 nM following the manufacturer’s guidelines.

Article Title: Endothelial cell-derived IGFBP7 suppresses angiogenesis and tumor progression in colorectal cancer via the VAPA-TGF-β1 pathway
Article Snippet: Tumor cells were treated with recombinant IGFBP7 protein (R&D Systems, Cat# 1334-B7) for 48 h. After treatment, the culture supernatants and cells were collected for subsequent experiments. .. IGFBP7 siRNA and negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China), and siRNA transfection of HMEC-1 cells and HUVEC cells was performed according to the manufacturer’s protocol. ..

Article Title: DMNQ induces ferroptosis and augments the efficacy of anti-PD-L1 immunotherapy in gastric cancer via the STAT3/SLC1A4 axis to mediate cysteine metabolism reprogramming.
Article Snippet: Antibodies specific for Ki67 (27309-1-AP; 1:2000 for immunohistochemistry), GZMB (13588-1-AP; 1:200 for immunofluorescence), and GAPDH (60004-1-Ig; 1:10000 for WB) were purchased from Proteintech (Wuhan, China). .. The STAT3 and SLC1A4 small interfering RNA (siRNA) or respective negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China). .. The siRNAs were transfected into cells utilizing OptiMEM and Lipofectamine 2000 transfection reagent (Invitrogen, CA, USA) at a concentration of 100 nM following the manufacturer's guidelines.

Knockdown:

Article Title: Targeting FGG alleviates cholestatic fibrosis by inhibiting hepatic stellate cell activation and regulating macrophage homeostasis.
Article Snippet: .. For gene knockdown, cells at 70–80% confluence were transfected with 50nM human FGG-specific siRNA or negative control (NC) siRNA (OBiO Technology), with or without concurrent stimulation by 5ng/mL TGF-β1 (MCE, Shanghai, China). ..

Small Interfering RNA:

Article Title: Helicobacter pylori -induced aberrant methylation of ID4 mediated by DNMT3B drives gastric cancer progression via DEC1-SHH signaling pathway
Article Snippet: .. The ID4 small interfering RNA (siRNA) or respective negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China). ..

Article Title: Helicobacter pylori -induced aberrant methylation of ID4 mediated by DNMT3B drives gastric cancer progression via DEC1-SHH signaling pathway
Article Snippet: .. The DNMT3B and KLF5 small interfering RNA (siRNA) or respective negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China). .. The siRNAs were transfected into cells utilizing Opti-MEM and Lipofectamine 2000 transfection reagent (Invitrogen, CA, USA) at a concentration of 100 nM following the manufacturer’s guidelines.

Article Title: DMNQ induces ferroptosis and augments the efficacy of anti-PD-L1 immunotherapy in gastric cancer via the STAT3/SLC1A4 axis to mediate cysteine metabolism reprogramming.
Article Snippet: Antibodies specific for Ki67 (27309-1-AP; 1:2000 for immunohistochemistry), GZMB (13588-1-AP; 1:200 for immunofluorescence), and GAPDH (60004-1-Ig; 1:10000 for WB) were purchased from Proteintech (Wuhan, China). .. The STAT3 and SLC1A4 small interfering RNA (siRNA) or respective negative control (NC) siRNA were purchased from Obio Technology (Shanghai, China). .. The siRNAs were transfected into cells utilizing OptiMEM and Lipofectamine 2000 transfection reagent (Invitrogen, CA, USA) at a concentration of 100 nM following the manufacturer's guidelines.



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